[BioC] miRNA

James W. MacDonald jmacdon at med.umich.edu
Fri Jul 8 16:09:14 CEST 2011


Hi Ayse,

Please read the posting guide:

http://www.bioconductor.org/help/mailing-list/posting-guide/

and then re-post with the sort of information we will need to help you.

Best,

Jim

On 7/7/2011 9:07 AM, Ayse Ulgen wrote:
> Hello,
>
> I did normalisation on my .CEL files using the rma package.
>
> Now, do I need to have a separate file ( with probe/microRNA names
> and expression values (not log2 transformed)) ?? Is this is the file
> that I created in R called RData?
>
> What about the log2 transformed expression values? How do I check
> that the expression values are low?What do I do in that case? How do
> I check?
>
> Thank you, A.
>
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-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
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