[BioC] How to use 'ebayes' for small samples?

[James W. MacDonald]

Hi Gundala,

On 4/15/2013 10:11 PM, Gundala Viswanath wrote:
> I have the following expression data
> from test and control, each contain two sample.
>
> test<- c(3922.89022881,4235.76026274)
> control<- c(3694.11962861,4369.97835686)
>
>
> I want to compute P-value using , to see if
> they are differentially expressed, using
> "ebayes"  http://rss.acs.unt.edu/Rdoc/library/limma/html/ebayes.html'
>
> What's the way to go about it?
> It's not clear from the help file.

You wouldn't use eBayes() for making a single comparison. And you 
wouldn't normally use unlogged data. And just looking at those data, I 
can tell you the p-value is going to be very close to 1.

Best,

Jim


>
>
>
> - G.V.
>
> 	[[alternative HTML version deleted]]
>
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-- 
James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
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