[BioC] RMA Normalization Nimblegen with adf.txt file

Piet Jones pietjones at gmail.com
Tue Jul 1 21:58:55 CEST 2014


Hi,

I am trying to normalize a nimblegen vitis vinifera microarray
experiment. The normal procedure I use is to use the pdInfoBuilder
package to create a R package from a xys file and a ndf file, I then
install and load the package followed by using the read.xysfiles, rma
and write.exprs methods in the oligo package.

My problem is that the particular experiment I need to analyse only
has a adf.txt file, I have searched in vain to find a way to convert
this to ndf. Does anyone know of a way to do this? Or is there some
alternative RMA normalization that can be done with nimblegen xys/pair
files and a adf.txt file?

Kind Regards,
Piet Jones



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