# [R] How to set up a Negative Exponential regression mixed effect model 'nlme' ?

Ronny Steen kestrel1978 at gmail.com
Thu Nov 17 23:13:48 CET 2016

```Hi,

I need help with fitting a non-linear mixed effects model (nested random
effect). I look at the relationship between 'wingbeat frequency' (beats per
sec) and 'wing length' in hummingbirds, and I will find a model with the
best fit.

I have posted the question at R-SIG-mixed-models list, but no answer so
far, therefore I give it a new try here.

I manage to fit Negative Natural Exponential regression and Assymptotic
(SSasymp-function) nonlinear regression, but I don't figure out how to fit
Negative Exponential regression (similar to nls {stats} function
'negexp.SSival').

*My question:* Could I get some help adapting the Negative Exponential
regression model (*'nls'*, without random effect) to a model with nested
random effect (*'nlme' *)?

library(nlme)

library(MASS)

Species2016.csv.csv?raw=1"))

plot(WBF\$WL,WBF\$Beat_freq,type="p",ylab="WBF")# Make a plot

#Negative Natural Exponential regression with nested random effect

fm1<-lme(Beat_freq ~ exp(-WL),

data = WBF,

random = ~ 1|Species/ID, method = "ML")

#Assymptotic (SSasymp-function) nonlinear regression with nested random
effect

fm2 <- nlme(Beat_freq ~ SSasymp(WL, Asym, R0, lrc),

data = WBF,

fixed = Asym + R0 + lrc ~ 1,

random = Asym ~ 1|Species/ID,

start = c(Asym = 27, R0 = 6000, lrc = 0.19))

#Negative Exponential regression - HELP NEEDED TO INCLUDE NESTED RANDOM
EFFECT ('nlme')

negexp<-selfStart(model = ~b0 + b1*exp(-x/th), initial =

negexp.SSival,parameters=c("b0","b1","th"),

template=function(x,b0,b1,th){})

fm3<-nls(Beat_freq~negexp(WL,B0,B1,theta),

data=WBF,control=list(maxiter =5000),trace=T)

Regards,

Ron

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