[BioC] Filtering from Affy
Rafael A. Irizarry
rafa@jhu.edu
Wed, 11 Dec 2002 14:02:23 -0500 (EST)
i highly recommend learning about the exprSet class and in
particluar the phenoData slot. a place to start is the
vignette for the Biobase package. Bioconductor also has a genefilter
package that you might find useful.
hope this helps,
-rafael
On Wed, 11 Dec 2002,
Michael Benjamin wrote:
> Hello-
>
> I have Affymetrix .cel files that I have read into R using the affy
> package. I am able to do normalization, transformation, and even
> cluster analysis. I cannot figure out how to do gene filtering
> operations-how do I tell R which samples I am interested in comparing to
> which other samples? I have two duplicate experiments that are vector
> controls, two duplicate experiments that are virally transfected, and
> one experiment that is the parental cell line. How do I compare
> differential gene expression between the vector controls and the virally
> transfected cell lines, for example?
>
> Thanks,
> Michael Benjamin, MD
>
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