[BioC] Affy digestion plots

Oliver Hartmann hartmann at mailer.uni-marburg.de
Fri Jul 18 15:18:24 MEST 2003


Dear list members,

I applied the digestion plot to two different Affy chips (library affy 
v. 1.1.5, hgu133a_1.2.6, hgu95a_1.1.0, R 1.6.1).
While for the U95Av2 the plots look like expected, I get a slope close 
to 1 for all chips on the U133A (see attached ppt slide, where identical 
biological samples were hybridised to both chips). I have observed 
similar things with some other chips (mouse or rat, can't remember right 
now but I can look it up if of interest).

Is there a bug in the function AffyRNAdeg() or is this due to the new 
chip design?

Thanks a lot

	-oli-

Script used:
deg <- AffyRNAdeg(data)
summaryAffyRNAdeg(deg)
plotAffyRNAdeg(deg)


-- 
Oliver Hartmann, Institute of Medical Biometry and Epidemiology
Philipps-University Marburg, Bunsenstr. 3, D-35037 Marburg
phone +49(0)6421 28 66514, fax +49(0)6421 28 68921
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